Molecular mapping of stem and leaf rust resistance in wheat |
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| Authors: | R R Khan H S Bariana B B Dholakia S V Naik M D Lagu A J Rathjen S Bhavani V S Gupta |
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| Institution: | (1) Plant Molecular Biology Unit, Biochemical Sciences Division, National Chemical Laboratory, Pune, 411008, India;(2) Plant Breeding Institute, Cobbitty, PMB11 Camden NSW2570, The University of Sydney, Sydney, Australia;(3) Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, SA5064, Australia;(4) Present address: Agriculture and Agri-food Canada, Pacific Agri-Food Research Centre, Summerland, VOH 1Z0, BC, Canada |
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| Abstract: | Stem rust caused by Puccinia graminis f. sp. tritici Eriks and Henn and leaf rust caused by Puccinia triticina Rob. ex Desm. are major constraints to wheat production worldwide. In the present study, F4-derived SSD population, developed from a cross between Australian cultivars ‘Schomburgk’ and ‘Yarralinka’, was used to identify
molecular markers linked to rust resistance genes Lr3a and Sr22. A total of 1,330 RAPD and 100 ISSR primers and 33 SSR primer pairs selected ob the basis of chromosomal locations of these
genes were used. The ISSR marker UBC 840540 was found to be linked with Lr3a in repulsion at a distance of 6.0 cM. Markers cfa2019 and cfa2123 flanked Sr22 at a distance of 5.9 cM (distal) and 6.0 cM (proximal), respectively. The use of these markers in combination would predict
the presence or absence of Sr22 in breeding populations. A previously identified PCR-based diagnostic marker STS638 linked to Lr20 was validated in this population. This marker showed a recombination value of 7.1 cM with Lr20. |
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