中国鄂温克族和达斡尔族人群中血小板膜糖蛋白Ib(GPIb)的多态性

研究旨在进一步探索同一人种的不同民族之间GPIb的多态性是否存在差异。血小板从84名健康鄂温克族，85名达斡尔族青年志愿采血分离而获得，血小板样品经SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）后，再转移至硝酸纤维素膜上（Western blotting)，用生物素化的素胚凝集素（WGA/Bio)偶联辣根过氧化酶复合物试剂盒（ABCkit)分步孵育，再以4-氯-1-萘酚显影，膜上呈现的深灰色带即是GPIbα链，其分子量分别为141、136、132、128kD，此即A、B、C、D四型，此为基因型，其中每两型构成一个表型，本研究证明，在我国鄂温克族人群中，等位基因A、B、C、D的频率分别为0.113,0.375,0.381,0.131;在达斡尔族中，A型为0.124，B型为0.406，C型为0.359。D型为0.112。经统计学分析。在这两个民族的人群之间，其GPIb各基因频率无显性差异。两个民族人群的GPIb表型经卡方检验也无显性差异。由此结果说明，鄂温克，达斡尔两个民族的人群之间，GPIb的多态性特征是相似的。

THE POLYMORPHISM OF PLATELET GLYCOPROTEIN Ib(GPIb) IN THE POPULATIONS OF CHINESE EWENKI AND DAUR NATIONALITIES

To study the differences of the GPIb polymorphism patternsbetween the Ewenki and Daur nationalities, Platelet samples were obtained from 169 healthy young Chinese volunteers, 84 Ewenki and 85 Daur; analysed by means of SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and followed by Western blotting. The membranes were incubate with biotinylated wheat germ agglutinin (WGA/Bio) and avidin-biotin horseradish peroxidase complex (ABC kit) respectively, then developed with 4-chloro-1-naphthol. The GPIbα chain was shown as dark gray bands on the nitrocellulose membrane. The molecular weights were 141, 136, 132, and 128 kD, which represented A, B, C and D genotypes respectively. Every two of the genotypes makes up a phenotype. The frequancy of the alleles A, B, C and D were 0.113, 0.375, 0.381 and 0.131 in Ewenkis; and 0.124, 0.406, 0.359 0.112 in Daurs. Statistical analysis showed no significant differences between these two nationalities, either in alleles A, B, C, D frequency or in their phenotype frequency. This result suggests that the GPIb polymorphisms are similar between Ewenki and Daur nationalities.