Vitellogenesis in the shrimp,Penaeus vannamei: in vitro studies of the isolated hepatopancreas and ovary |
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Affiliation: | 1. Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China;2. Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science, Nanchang, Jiangxi 330200, China;1. Institute for mining and metallurgical machinery, RWTH Aachen University, Aachen, Germany (Tel: +49 241-80-90744);7. Institute for mining and metallurgical machinery, RWTH Aachen University, Aachen, Germany (Tel: +49 241-80-90756);71. Institute for mining and metallurgical machinery, RWTH Aachen University, Aachen, Germany;77. Institute for mining and metallurgical machinery, RWTH Aachen University, Aachen, Germany (Tel: +49 241-80-95680) |
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Abstract: |
- 1.1. Yolk proteins were isolated from ovaries of the shrimp Penaeus vannamei and used as an antigen for antibody production in rabbits.
- 2.2. Protein synthesis was measured for both the hepatopancreas and the ovary in vitro, and proteins present in both tissues were immunoreactive with the antibodies.
- 3.3. Extracts of shrimp eyestalks inhibited in vitro protein synthesis by both tissues. The inhibitory factor from the eyestalks was heat stable and had a molecular weight of 3300 daltons.
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