Inhibition of protein synthesis in a polyribosome-dependent cell-free system by a specific ribonuclease prepared from the follicles of two different stages of development of the silkmothAntherea pernyi |
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Authors: | Emmanuel G Fragoulis Peter Traub |
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Institution: | (1) Department of Biochemistry, Cell and Molecular Biology and Genetics, University of Athens, Panepistemiopolis Kouponia, 157.01 Athens, Greece;(2) Max Planck Institute for Cell Biology, Rosenhoff, D-6802 Ladenburg, Germany |
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Abstract: | Summary Crude, cell-free protein-synthesizing systems were prepared from follicles of two different stages of development in the ovariole of the silkmothAntherea pernyi. The efficiency of the translation of natural and synthetic mRNAs in these systems was compared with that in a cell-free wheat germ system. A postmitochondrial extract (S-30) from the follicles almost completely inhibited protein synthesis in a polyribosome-dependent, cell-free systems. A specific ribonuclease, obtained from the post mitochondrial extract by ammonium sulphate precipitation, heat denaturation and DEAE-cellulose chromatography, inhibited polyribosome-dependent protein synthesis. The effect of this specific ribonuclease on the structural integrity of radioactive RNAs and ribosomal subunits, which were isolated from Ehrlich ascites tumor cells, was also studied. |
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Keywords: | Protein synthesis Development Antherea pernyi Follicles Specific ribonuclease |
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