首页 | 本学科首页   官方微博 | 高级检索  
     


Identification and functional characterization of phenylglycine biosynthetic genes involved in pristinamycin biosynthesis in Streptomyces pristinaespiralis
Authors:Mast Yvonne J  Wohlleben Wolfgang  Schinko Eva
Affiliation:Mikrobiologie/Biotechnologie, Interfakultäres Institut für Mikrobiologie und Infektionsmedizin, Fakultät für Biologie, Eberhard Karls Universität Tübingen, Auf der Morgenstelle 28, D-72076 Tübingen, Germany
Abstract:Pristinamycin I (PI), a streptogramin type B antibiotic produced by Streptomyces pristinaespiralis, contains the aproteinogenic amino acid l-phenylglycine. Recent sequence analysis led to the identification of a set of putative phenylglycine biosynthetic genes. Successive inactivation of the individual genes resulted in a loss of PI production. Production was restored by supplementation with externally added l-phenylglycine, which demonstrates that these genes are involved in phenylglycine biosynthesis and thus probably disclosing the last essential pristinamycin biosynthetic genes. Finally, a putative pathway for phenylglycine synthesis is proposed.
Keywords:Dpg, 3,5-dihydroxyphenylglycine   Hpg, 4-hydroxyphenylglycine   HPLC, high performance liquid chromatography   HPLC-DAD, HPLC-diode array detection   MRSA, methicillin-resistant Staphylococcus aureus   NRPS, nonribosomal peptide synthetase   ORF, open reading frame   PI, pristinamycin I   PII, pristinamycin II   PCR, polymerase chain reaction   Phg, phenylglycine   pgl, designation of the phenylglycine genes   VREF, vancomycin-resistant Enterococcus faecium
本文献已被 ScienceDirect PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号