Theβ-subunit of human chorionic gonadotropin containsN-glycosidic trisialo tri- and tri′-antennary carbohydrate chains |
| |
Authors: | Jan B L Damm Hans Voshol Karl Hård Johannis P Kamerling Gijs W K Van Dedem Johannes F G Vliegenthart |
| |
Institution: | (1) Department of Bio-Organic Chemistry, Utrecht University, Transitorium III, P.O. Box 80.075, NL-3508 TB Utrecht, The Netherlands;(2) Diosynth B.V., P.O. Box 20, NL-5340 BH Oss, The Netherlands |
| |
Abstract: | TheN-linked carbohydrate chains of the-subunit of highly purified urinary human chorionic gonadotropin have been re-investigated. The oligosaccharides were released enzymatically by peptide-N
4-(N-acetyl--glucosaminyl)asparagine amidase-F, and fractionated by a combination of FPLC and HPLC. As a result of the application of improved fractionation methods, apart from the earlier reported carbohydrate chains, also small amounts of trisialo tri- and tri-antennary oligosaccharides were found. The primary structures of the latter carbohydrate chains have been determined by 500-MHz1H-NMR spectroscopy to beAbbreviations hCG
human chorionic gonadotropin
- hCG-
-subunit
- hCG-
-subunit
- PNGase-F
peptide-N
4-(N-acetyl--glucosaminyl)asparagine amidase-F (E.C. 3.5.1.52)
- endo-F
endo--N-acetylglucosaminidase-F (E.C. 3.2.1.96)
- SDS
sodium dodecyl sulphate
- PAGE
polyacrylamide gel electrophoresis
- CBB
coomassie brilliant blue R 250
- GlcNAc
N-acetylglucosamine
- NeuAc
N-acetylneuraminic acid
- Man
mannose
- Gal
galactose
- Fuc
fucose |
| |
Keywords: | hCG PNGase-F FPLC HPLC 1
H-NMR |
本文献已被 SpringerLink 等数据库收录! |
|