Long-term study of somatic embryogenesis from anthers and ovaries of 12 grapevine (<Emphasis Type="Italic">Vitis</Emphasis> sp.) genotypes |
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Authors: | Email author" target="_blank">Julie?R?KikkertEmail author Michael?J?Striem José?R?Vidal Patricia?G?Wallace John?Barnard Bruce?I?Reisch |
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Institution: | (1) Department of Horticultural Sciences, New York State Agricultural Experiment Station, 633 W. North Street, 14 456-0462 Geneva, NY;(2) Computer Center, Cornell University, New York State Agricultural Experiment Station, 633 W. North Street, 14456-0462 Geneva, NY;(3) Present address: Sun World International Inc., 16350 Driver Road, 93 308-9733 Bakersfield, CA |
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Abstract: | Summary Anthers and ovaries of six grapevine cultivars (three Vitis vinifera L., two V × Labruscana L. H. Bailey, and one complex hybrid) were extracted from flower buds over 2 yr and cultured on three media reported to promote
somatic embryogenesis in Vitis tissues. The highest percent embryogenesis from the hybrid ‘Chancellor’ and V. vinifera ‘Chardonnay’, ‘Merlot’, and ‘Pinot Noir’ occurred on medium C Nitsch and Nitsch, 1969, basal medium with 3.0% (w/v) sucrose,
0.01% (w/v) inositol. 0.3% (w/v) Phytagel, 2.5 μM 2.4-dichlorophenoxyacetic acid, 2.5μM β-naphthoxyacetic acid, 5.0μM N-(2-chloro-4-pyridyl)-N′-phenylurea, and 0.05% (w/v) glutamine]. Regardless of the media, the labrusca cultivars ‘Concord’ and ‘Niagara’ produced
soft non-embryogenic callus that was sometimes mixed with well-developed somatic embryos. Nine vinifera genotypes were further
tested for several different years on medium C. Embryogenic cultures suitable for transformation were obtained from all genotypes
in more than 1 yr. The average percent embryogenesis from ovaries was 7-fold higher than from anthers. There was significant
annual variation in percent embryogenesis, demonstrating the need for media comparisons to be replicated for more than one
season. Suspension cultures suitable for use in genetic transformation were initiated from ‘Chardonnay’, ‘Merlot,’ and ‘Pinot
Noir’ pro-embryogenic masses. ‘Chardonnay’ suspension cultures plated and grown under conditions developed for recovery of
plants after biolistic transformation yielded approximately 500 non-transformed embryos per plate after 4 mo. of culture,
with 68.6% of the embryos converting to plants. This is the first reported protocol for embryogenesis from ‘Concord,’ ‘Cabernet
Franc,’ and ‘Pinot Noir’ grapevines. |
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Keywords: | adventitions embryos in vitro culture regeneration tissue culture Vitis vinifera Vitis × Labruscana |
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