In vitro growth of tick tissues (Rhipicephalus appendiculatus neumann, 1901) |
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| Affiliation: | 1. Chemical Engineering Department, Loughborough University, LE11 3TU, UK;2. Department of Infection, Immunity and Inflammation, University of Leicester, University Road, Leicester LE1 7RH, UK;3. Department of Mathematics and Computer Science, Liverpool Hope University, Hope Park, Liverpool L16 9JD, UK |
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| Abstract: | - 1.1. The medium, in which initial outgrowths and long survival of several kinds of tick tissue cells occurred, contained Hanks' balanced salt solution, amino acids and vitamins of Eagle's basal medium together with 20% ox serum.
- 2.2. Explants, comprising body contents of nymphs and adult ticks (Rhipicephalus appendiculatus) were cultured in Leighton culture tubes.
- 3.3. Nymphs, dissected 4 to 7 days after engorgement, seemed to be most satisfactory for the preparation of tick tissue cultures.
- 4.4. Initial movements in tissues and outgrowth of cells frequently occurred within 24 hours in vitro.
- 5.5. Four cell types were recognized in the outgrowths. Mitotic changes in the outgrowths were also observed among all cell types.
- 6.6. Some of the explant tissues commonly survived in vitro over 60 days and tissue movements and cell outgrowths were observed for 173 and 175 days respectively.
- 7.7. Explant transfers (subculturing) were carried out with some success. In one instance outgrowths continued for 100 days.
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