影响叶螨磷酸酯酶活性的四因子数学模型

应用二次回归通用旋转组合设计，组建了影响叶螨磷酸酯酶（酸性和碱性）活性的四因子（缓冲液Ph值X₁、温浴时间X₂、反应温度X₃、底物浓度X₄）数学模型: Y_酸性=0.456380+0.107889X₂+0.069027X₃-0.026836X₁²-0.030794X₃², F=24.98，P<0.01；Y_碱性=0.267286-0.200736X₁+0.049541X₂+0.030930X₃-.049063X₁X₂+0.053585X₁²-0.049665X₂^2, F=57.68，P<0.01。结果表明，温浴时间是影响叶螨酸性磷酸酯酶活性的关键因子，在缓冲液pH 4.4、底物浓度8.5×10^-3 mol/L、42℃温浴40 min测得该酶活性最强。影响碱性磷酸酯酶活性的关键因子则是缓冲液pH值，pH 9.0、37℃恒温30 min、底物7.5×10^-3 mol/L的条件下，光密度值最大。两种酶的最大吸收峰波长为405 nm。

MATHEMATICAL MODEL FOR THE FACTORS AFFECTING PHOSPHATASE ACTIVITY IN TETRANYCHUS (ACARI:TETRANYCHIDAE)

The regression method was adopted to establish mathematical models for the selection of most effective combination of pH value of buffer, constant temperature, reaction time and substrate concentration, that are four major factors affecting activity of phosphatase, either acidic or alkaline, in Tetranychus cinnabarinus. The models are: Y_acidic=0.456380+0.107889X²+0.069027X³-0.026836X₁²-0.030794X₃², F=24.98，P<0.01; Y_alkaline=0.267286-0.200736X₁+0.049541X₂+0.030930X₃-0.049063X₁X₂+0.053585X₁²-0.049665X₂², F=57.68, P<0.01. It reveals that the acid phosphatase has the highest activity under the combination of buffer pH 4.4, temperature 42℃, reaction time 42 minutes and substrate concentration 8.5×10^-3 mol/L. The reaction time is a key factor affecting acid phosphatase. The key factor affecting alkaline phosphatase is the buffer pH. The best combination is pH 9.0, 37℃, 30 minutes and 7.5×10^-3 mol/L.