Novel arbekacin- and amikacin-modifying enzyme of methicillin-resistant Staphylococcus aureus |
| |
Authors: | Fujimura S Tokue Y Takahashi H Kobayashi T Gomi K Abe T Nukiwa T Watanabe A |
| |
Institution: | Department of Respiratory Oncology and Molecular Medicine, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan. hujimura@mail.sp.myu.ac.jp |
| |
Abstract: | An aminoglycoside-modifying enzyme in arbekacin-resistant methicillin-resistant Staphylococcus aureus (MRSA), exhibiting 4'-N-acetylation, was examined. Although the MRSA strain with AAC(4') had no AAC(6')-APH(2") activity, a DNA fragment of the AAC(6')-APH(2") gene was amplified by PCR and the purified N-terminal 30-amino acid sequence of this AAC(4') was identical to AAC(6')-APH(2"). Direct DNA sequencing of this 'silent' AAC(6')-APH(2") gene revealed a single point mutation leading to a substitution of Gly for Asp80, through which the secondary structure is affected. A change in protein conformation could lead to a cleavage and a change of the enzymatic activity. We propose a new aminoglycoside-resistance mediated by AAC(4') is caused by a mutation-modified AAC(6')-APH(2"). |
| |
Keywords: | Aminoglycoside resistance Arbekacin Amikacin Aminoglycoside acetyltransferase Methicillin-resistant Staphylococcus aureus |
本文献已被 PubMed 等数据库收录! |
|