Kinetic study in the transient phase of the suicide inactivation of frog epidermis tyrosinase |
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Authors: | Jos Tudela Francisco Garcia Cnovas Ramn Varn Mercedes Jimnez Francisco Garcia-Carmona Jos A Lozano |
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Institution: | Departamento de Bioquimica y Biologia Molecular, Universidad de Murcia, Spain. |
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Abstract: | This paper deals with the kinetic study of a multisubstrate mechanism with enzyme inactivation induced by a suicide substrate. A transient phase approach has been developed that enables the deduction of explicit equations of product concentration vs. time. From these equations kinetic constants which characterize the suicide substrate can be obtained. This study with tyrosinase enzyme, which acts on L-dopa and catechol allowed us to determine the corresponding kinetic parameters, indicating that catechol is about 8-times more powerful as a suicide substrate than is L-dopa. |
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Keywords: | Enzyme kinetics Enzyme inactivation Suicide substrate -Dopa Catechol Tyrosinase Transient phase |
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