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Qualitative studies of pneumococcal polysaccharides by infrared spectrophotometry
Authors:LEVINE S  STEVENSON H J  KABLER P W
Affiliation:1. Cool Climate Oenology and Viticulture Institute (CCOVI), Brock University, St. Catharines, ON, Canada;2. Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Padova, Italy;3. Wine Australia, Industry House, National Wine Centre, Adelaide, SA, Australia;4. University of Reims Champagne-Ardenne, Laboratoire d’Oenologie, Reims, France;5. University de Haute-Alsace, LVBE, Colmar, France
Abstract:
The infrared spectra of pneumococcal polysaccharides are presented. The spectra of samples of the same type prepared by different methods are very similar. The preparations examined are divided into four groups depending on whether the spectra exhibit amide bands, carboxylate bands, both, or neither. A clear distinction can be made spectrally between O-acetyl and N-acetyl.All of 57 types and subtypes could be distinguished by their spectra except one pair. The spectral comparison of serologically related types is discussed.
Keywords:
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