| HFRSV内影像型抗独特型人单抗重链可变区基因克隆 |
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| 引用本文： | 高磊,陈苏民,陈南春,赵忠良,刘新平,杨萍,杨安钢,米力,崔运昌. HFRSV内影像型抗独特型人单抗重链可变区基因克隆[J]. 中国生物化学与分子生物学报, 1994, 10(3): 274-278 |
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| 作者姓名： | 高磊 陈苏民 陈南春 赵忠良 刘新平 杨萍 杨安钢 米力 崔运昌 |
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| 作者单位： | 第四军医大学分子生物学研究所生化研究室单克隆研究室 |
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| 摘 要： | 利用反转录－ＰＣＲ方法,从分泌肾综合症出血热病毒（ＨＦＲＳＶ）内影像型抗独特型人单抗杂交瘤细胞系（Ｃ８）中,成功地克隆了抗ＨＦＲＳＶ１ｄ人单抗重链可变区基因,并将此基因重组入Ｍ１３噬菌体ＤＮＡ中,测定了此重链可变区基因全序列。经计算机分析,基因全长共３５１ｂｐ、编码１１７个氨基酸,氨基酸序列同源性分析发现所推得的该单抗ＣＤＲ２区与ＨＦＲＳＶＧ２蛋白Ｃ端有同源区,此区可能具有较强的抗原性。
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| 关 键 词： | 抗独特型抗体 PCR 可变区基因 肾综合症出血热病毒（HFRSV）, |
| 收稿时间： | 1994-06-20 |
Gene Cloning of the Heavy-Chain Variable Region of an Internal Image Human Monoclonal Anti-idiotype Antibody against Hemorrhagic Fever Virus |
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| Gao,Lei,Chen,Su-min,Chen,Nan-chun,Zhao,Zhong-ling,Liu,Xinping,Yang,Ping,Yang,An-gang,Mi,Li,Cui,Yun-chang. Gene Cloning of the Heavy-Chain Variable Region of an Internal Image Human Monoclonal Anti-idiotype Antibody against Hemorrhagic Fever Virus[J]. Chinese Journal of Biochemistry and Molecular Biology, 1994, 10(3): 274-278 |
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| Authors: | Gao Lei Chen Su-min Chen Nan-chun Zhao Zhong-ling Liu Xinping Yang Ping Yang An-gang Mi Li Cui Yun-chang |
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| Affiliation: | (Department of Biochemistry,Section of Monoclonal Antibody, Institute of Molecular Biology, The 4th Military Medical University, Xian 710033 |
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| Abstract: | The heavy-chain variable region of an internal image human monoclonal antiidiotype antibody against Hemorrhagic Fever Virus was cloned from the hybridoma cell line (C8) by Reverse transcription-PCR method. The nucleotide sequence of the amplified DNA was determined after subcloning of PCR fragment in M13 vector. By computer analysis, the VH region consists of 351 base pairs encoding 117 amino acids. The comparison of amino acid sequences between the deduced CS VH peptide and HFRSV proteins revealed high homology between the CDRZ of the VH and the GZ of HFRSV. The antigenicity of this region was discussed. |
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| Keywords: | Anti-idiotype antibody RCR Variable region HFRSV |
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