中华卵索线虫雌雄成虫可溶性蛋白双向电泳分析

昆虫寄生线虫 (又称虫生线虫、昆虫病原线虫)是本世纪发展起来的一种有潜能的生物防治因子 ,它寄主范围广、能主动寻找寄主、对人畜及环境安全无毒。中华卵索线虫是昆虫寄生线虫的一种 ,由我国学者发现并命名的新种 (陈果等 ,1 9 91 ) ,具有广泛的寄主范围 ,可杀灭粘虫 (任慧芳等 ,1 989)、烟青虫、烟蚜 (侯茂林等 ,2 0 0 2 )及棉铃虫 (陈果 ,1 994)等危害十分严重的害虫 ,其寄生率等于宿主的死亡率。因此在生物防治手段日益突出的今天 ,中华卵索线虫无疑有着广泛的应用前景。然而 ,在线虫培养的过程中 ,无论是体内培养还是体外培养 ,我们发…

Preliminary analysis on dissoluble protein of Ovomermis sinensis adults by 2-D PAGE

Ovomermis sinensis (Nematode,Mermithidae) is a parasitic nematode of ar myworm. But during its culture in vitro and rearing in vivo, we found that we co uld not attain the suitable proportion of male and female nematodes. In order to disclose the reason why the number of the filial generation was imbalanced betw een two sexes and get basic data on genetic development, we analyzed dissoluble proteins of Ovomermis sinensis imagoes with electrophoresis. Firstly, throug h SD S-PAGE, we found more than three specific proteins were detected in female imag o with the approximate molecular weight of 15-16 kD, 21-24 kD, 72-76 kD and on e abou t 26 kD specific protein in male imago. Secondly, we used a new improved two-di me nsional electrophoresis method to analyze the proteins and found that the result acco rds with those of SDS-PAGE, which showed that this 2-DE method is practicable. A lso, in this 2-DE, proteins can be well separated and plenty of different pr oteins can be clearly displayed .