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Silencing of Kv4.1 potassium channels inhibits cell proliferation of tumorigenic human mammary epithelial cells |
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| Authors: | Soo Hwa Jang,Seong-Geun Hong,Young Min Bae,Scott M. O&rsquo Grady,Kyung-Sun Kang,So Yeong Lee |
| Affiliation: | a Laboratories of Veterinary Pharmacology, College of Veterinary Medicine, Seoul National University, San 56-1 Sillim-Dong Kwanak-Gu, Seoul 151-742, South Korea b Veterinary Public Health, College of Veterinary Medicine, Seoul National University, San 56-1 Sillim-Dong Kwanak-Gu, Seoul, South Korea c Department of Food and Nutrition, College of Human Ecology, Chung-Ang University, Anseong, Gyeonggi, South Korea d Department of Physiology, College of Medicine, Gyeongsang National University, Jinju, South Korea e Department of Physiology, College of Medicine, Konkuk University, Seoul, South Korea f Department of Physiology, 495 Animal Science/Veterinary Medicine Bldg., St. Paul, University of Minnesota, MN, USA g Research Institute and Hospital, National Cancer Center, Goyang, Gyeonggi, South Korea |
| Abstract: | Potassium channel activity has been shown to facilitate cell proliferation in cancer cells. In the present study, the role of Kv4.1 channels in immortal and tumorigenic human mammary epithelial cells was investigated. Kv4.1 protein expression was positively correlated with tumorigenicity. Moreover, transfection with siRNAs targeting Kv4.1 mRNA suppressed proliferation of tumorigenic mammary epithelial cells. Experiments using mRNA isolated from human breast cancer tissues revealed that the level of Kv4.1 mRNA expression varied depending on the stage of the tumor. Kv4.1 protein expression increased during stages T2 and T3 compared to normal tissue. These results demonstrated that Kv4.1 plays a role in proliferation of tumorigenic human mammary epithelial cells. In addition, elevated Kv4.1 expression may be useful as a diagnostic marker for staging mammary tumors and selective blockers of Kv4.1 may serve to suppress tumor cell proliferation. |
| Keywords: | Voltage-gated K+ channels Breast cancer Cell growth |
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