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Frequent overexpression of CADM1/IGSF4 in lung adenocarcinoma
Authors:Yuka Kitamura  Miho Tanaka  Chiho Muramatsu  Yasushi Akahori  Hiroyuki Tsuda  Yoshinobu Hattori
Affiliation:a Department of Surgery, School of Medicine, Fujita Health University, Toyoake, Aichi 470-1192, Japan
b Division of Antibody Project, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470-1192, Japan
c Department of Internal Medicine, School of Medicine, Fujita Health University, Toyoake, Aichi 470-1192, Japan
d 21st Century COE Research Center, Fujita Health University, Toyoake, Aichi 470-1192, Japan
e Department of Toxicology, Graduate School of Medical Sciences, Nagoya City University, Kawazumi, Mizuho, Nagoya 467-8601, Japan
Abstract:We reported comprehensive screening for antigens (Ags) overexpressed on various carcinomas via isolation of human monoclonal antibodies (mAbs) that may be therapeutic in a previous paper (Proc. Natl. Acad. Sci. USA 105, 7287-7292, 2008). Twenty-one distinct Ags highly expressed on several carcinomas were identified and 356 mAbs with unique sequences turned out to bind to one of the 21 Ags. Among them CADM1/IGSF4 which had been originally referred to as tumor suppressor lung cancer 1 (TSLC1) was included. Therefore we examined the expression of CADM1 in lung cancers in this study. Eight different anti CADM1 mAbs were used for immunohistochemical analysis of 29 fresh lung cancer specimens. Staining patterns were categorized to six groups based on the extent of positive staining and the localization of stained portions. While overexpression of CADM1 was observed on the cell surface of adenocarcinomas at a high frequency, around 60%, positive stainings were rarely observed on that of other lung carcinomas including squamous cell carcinomas. Moreover, some clones among the eight mAbs gave different staining patterns from those by the other clones against the same fresh specimen, suggesting presence of variant forms of CADM1 differentiated by mAbs.
Keywords:Ab, antibody   Ag, antigen   ELISA, enzyme-linked immunosorbent assay   FCM, flow cytometry   HUVEC, human umbilical vein endothelial cell   ICOS, isolation of Ag/Ab complexes through organic solvent   IHC, immunohistochemistry   IP, immunoprecipitation   mAb, monoclonal Ab   MS, mass spectrometry   TAA, tumor-associated Ag   TSLC, tumor suppressor lung cancer
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