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The MAP1-LC3 conjugation system is involved in lipid droplet formation |
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| Authors: | Masahiro Shibata Kentaro Yoshimura Norihiko Furuya Takashi Ueno Hiroyuki Arai Eiki Kominami Yasuo Uchiyama |
| Affiliation: | a Department of Cell Biology and Neuroscience, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan b Division of Gross Anatomy and Morphogenesis, Department of Regenerative and Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8510, Japan c Department of Biochemistry, Juntendo University School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan d Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, Bunkyo-ku, Tokyo 113-8421, Japan e Laboratory of Frontier Science, Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo 113-8613, Japan f Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Japan Bunkyo-ku, Tokyo 113-0033, Japan |
| Abstract: | Lipid droplets (LDs) are ubiquitous in eukaryotic cells, while excess free fatty acids and glucose in plasma are converted to triacylglycerol (TAG) and stored as LDs. However, the mechanism for the generation and growth of LDs in cells is largely unknown. We show here that the LC3 lipidation system essential for macroautophagy is involved in LD formation. LD formation accompanied by accumulation of TAG induced by starvation was largely suppressed in the hepatocytes that cannot execute autophagy. Under starvation conditions, LDs in addition to autophagosomes were abundantly formed in the cytoplasm of these tissue cells. Moreover, LC3 was localized on the surface of LDs and LC3-II (lipidation form) was fractionated to a perilipin (LD marker)-positive lipid fraction from the starved liver. Taken together, these results indicate that the LC3 conjugation system is critically involved in lipid metabolism via LD formation. |
| Keywords: | Lipid droplets Autophagy Autophagosomes LC3 Triacylglycerol Liver Heart Atg7 Microtubule associated protein 1A/1B light chain 3 |
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