周龄和激素水平对长爪沙鼠超数排卵效果的影响

目的探讨不同周龄和激素水平对长爪沙鼠超数排卵效果的影响,以期确定长爪沙鼠最佳超排周龄和激素使用剂量。方法腹腔注射10 IU PMSG/HCG对4～18周龄8个年龄段的雌性长爪沙鼠进行超数排卵,末次注射16～17 h内对各组动物卵母细胞计数,确定最佳超排周龄后,对该年龄动物以5、10、15 IU3个剂量水平腹腔注射PMSG/HCG,观察各组动物的卵母细胞计数差异。结果与其它周龄组相比,6周龄组长爪沙鼠超数排卵后的卵母细胞数最多,各组间有统计学意义（P〈0.05）,而5、10、15IU等3个剂量组的超排效果也有一定的差异,10 IU组数量最高。结论对长爪沙鼠而言,采用10 IU激素注射和6周龄的动物进行超数排卵,获得的卵母细胞数量最多而且超排效果稳定性。

Effect of Week-Age and Hormonal Level on Superovulation in Mongolian Gerbils

Objective To investigate the influence of different week-age and the hormonal level on superovulation in Mongolian gerbils.Methods Eight groups of Mongolian gerbils at different age of 4-18 weeks were induced to superovulate by intraperitoneal injection of 10 IU PMSG/HCG,respectively.At 16-17 h after the last injection,the amounts of eggs of animals in different groups were counted and analyzed to determine the best week-age of superovulation.Superovulation was induced in the animals of best week-age by intraperitoneal injection of 5,10,15 IU PMSG/HCG to analyze the different amounts of eggs.Results Compared with other groups,the amount of eggs obtained in gerbils at age of 6 weeks is highest and there were significant differences among all groups（P〈0.05）.The amounts of eggs obtained in groups of different PMSG/HCG doses were significantly different and that of the 10 IU group was highest.Conclusion The age of 6 weeks and the dose of 10 IU PMSG/HCG are the best ages and effective dose to induce superovulation in Mongolian gerbils.