亚健康状态大鼠的神经-免疫-内分泌机制的研究

目的探讨亚健康状态大鼠的神经-免疫-内分泌的机制。方法取雄性SPF级Wistar大鼠36只,按体重随机分成6组,即多因素组（MF）、多因素干预组（MFT）、热水游泳组（WS）、睡眠不足组（SD）、单纯束缚组（PC）和正常对照组（C）,每组6只。分别采用热水游泳、饮食限制、睡眠剥夺、束缚等方式建立亚健康大鼠模型,多因素干预组每日经口给予300 mg/kg的抗衰老片,在造模5 d后处理动物,并取大鼠血,分别测定血清5-羟色胺（5-HT）、多巴胺（DA）、甲状腺激素T3和T4、睾酮（T）、皮质酮（CORT）、促肾上腺皮质激素（ACTH）、白介素IL-1β、IL-2、IL-6、IL-8和IFN-γ含量以及T淋巴细胞亚群的变化,并取脾脏测定T、B淋巴细胞增殖能力和NK细胞活性变化。结果 （1）与正常对照组比,①造模结束后多因素组大鼠血清5-HT明显降低（P〈0.05）,而热水游泳组和单纯束缚组5-HT含量却升高显著（P〈0.01）,且睡眠不足组和单纯束缚组血清DA含量降低显著（P〈0.05）;同时各亚健康模型大鼠的血清T3、T4、CORT和ACTH含量均显著升高（P〈0.01）,T/C比值降低显著（P〈0.01）,且睡眠不足组血清T降低明显（P〈0.05）;②造模结束后多因素组的大鼠IL-8含量降低显著（P〈0.01）,而热水游泳组和单纯束缚组IL-1β、IL-2、IL-6、IL-8和IFN-γ含量以及睡眠不足组IL-1β、IL-2、IL-6含量均显著升高（P〈0.05,P〈0.01）,同时显著降低各亚健康模型大鼠淋巴细胞增殖转化能力和T淋巴细胞亚群CD3＋、CD4＋和CD4＋/CD8＋比值和NK细胞活性（P〈0.05,P〈0.01）。（2）与多因素组比,抗衰老片干预后多因素干预组大鼠血清DA含量明显升高（P〈0.05）,并能显著提高机体T淋巴细胞亚群CD3＋、CD4＋数目和CD4＋/CD8＋比值（P〈0.01）,降低血清CORT含量。结论亚健康状态能引起大鼠HPA轴反应性失衡,皮质醇释放过度,引起内分泌的紊乱,并能引起淋巴细胞功能降低,出现明显机体免疫抑制现象;而抗衰老片具有抗应激,提高T细胞免疫功能,改善情绪和内分泌紊乱的作用,从而达到改善亚健康体质的目的。

Study on the Mechanism of Neuro-Immuno-Endocrine System of Rats in Sub-Health State

Objective To explore the mechanism of neuro-immuno-endocrine system of rats in sub-health state.Methods Thirty-six male SPF Wistar rats were randomly divided into six groups： multi-factor group（MF）,multifactor treatment group（MFT）,warm water swimming group（WS）,sleep deficiency group（SD）,purely constraint group（PC） and control group（C）,six animals in each group.Respectively,using warm water swimming,dietary restrictions,sleep deprivation and constraint to establish sub-health rat models,and daily orally administered 300 mg/kg anti-aging tablet in the MFT group.At 5 days after modeling,the levels of serum 5-HT,DA,T3,T4,T,CORT,ACTH,IL-1β,IL-2,IL-6,IL-8,IFN-γ and T lymphocyte subsets were detected,and changes of T and B lymphocyte proliferation and NK cell activity in the spleen were also determined.Results（1） Compared with the control group,① Serum 5-HT in the MF group was significantly reduced（P〈0.05）,while 5-HT in the WS and PC groups was significantly increased（P〈0.01）,and the serum DA content in the SD and PC groups was significantly decreased（P〈0.05）.At the same time,the serum T3,T4,CORT and ACTH contents in each sub-health model rats were significantly increased（P〈0.01）,T/C ratio was significantly decreased（P〈0.01）,and the serum T in the SD group was significantly lowered（P〈0.05）;② Sserum IL-8 content in the MF group was significantly reduced（P〈0.01）,while the serum IL-1β,IL-2,IL-6,IL-8 and IFN-γ levels in the WS and PC groups,and IL-1β,IL-2 and IL-6 levels in the SD group were all significantly increased（P〈0.05,P〈0.01）,and the lymphocyte proliferation and T lymphocyte subsets CD3＋,CD4＋,CD4＋/CD8＋ ratio and NK cell activity were significantly reduced in all sub-health model rats（P〈0.05,P〈0.01）.（2） Compared with the MF group,the administration of anti-aging tablets induced significantly increased serum DA content in the MFT group（P〈0.05）,and significantly improved the T lymphocyte subsets CD3＋,CD4＋,CD4＋/CD8＋ ratio（P〈0.01）,and lowered serum CORT content.Conclusions Sub-health state can lead to imbalances of the rat HPA axis,excessive cortisol release,endocrine disorders,reduced lymphocyte function and immune inhibition,while anti-aging tablets have anti-stress effects,enhancing T cell immune function and ameliorating emotional and endocrine disorders,so as to achieve the purpose of improving the sub-healthy physique.