Chemically induced viral resistance in single preimplantation bovine embryos. |
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| Authors: | M S Thomson R C Bird D A Stringfellow C R Rossi L H Lauerman |
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| Affiliation: | Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849. |
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| Abstract: | Viral mRNA encoding vesicular stomatitis virus glycoprotein sequences was detected and quantitated using a DNA-hybridization dot-blot technique. This assay was employed to determine if the synthetic double-stranded polynucleotide complex of polyriboinosinic-polyribocytidylic acid would elicit viral resistance in vitro in single day 9 bovine embryos. The levels of viral mRNA were assayed in 4 groups of bovine embryos: unexposed, virus-exposed, polynucleotide-treated, and virus plus polynucleotide treated. Reduced quantities of viral mRNA in single polynucleotide treated embryos demonstrated that resistance to viral infection was induced in day 9 bovine embryos. |
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