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| 糙皮侧耳生长发育过程中漆酶基因家族的表达研究 | |
| 引用本文： | 卓睿,马富英,周帅,张晓昱. 糙皮侧耳生长发育过程中漆酶基因家族的表达研究[J]. 菌物学报, 2015, 34(4): 712-716. DOI: 10.13346/j.mycosystema.150075 |
| 作者姓名： | 卓睿 马富英 周帅 张晓昱 |
| 作者单位： | 华中科技大学生命科学与技术学院 湖北 武汉 430074 |
| 基金项目： | 国家重点基础研究发展计划（2014CB138301） |
| 摘 要： | 漆酶参与木质素的降解和食用菌的生长发育。为了明晰漆酶基因在糙皮侧耳生长发育过程中的作用,本文采用real-time PCR检测了11条漆酶基因及Lacc2的小亚基sspoxa3在糙皮侧耳生长发育不同阶段的表达。其中lacc6和sspoxa3在整个生长发育阶段表达量均较高;lacc12随着原基的分化和子实体的形成大量表达,与成菇过程有关。lacc4,lacc7和lacc11在原基分化期高表达,与原基的分化有关。lacc2,lacc3和lacc8在成熟子实体阶段表达量显著上升,与子实体的分化和成熟有关。 |
| 关 键 词： | 食用菌 漆酶 原基 子实体 实时定量PCR |
| 收稿时间： | 2015-03-02 |
Expression of laccase gene family in different development stages of Pleurotus ostreatus |
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| Rui ZHUO,Fu-Ying MA,Shuai ZHOU,Xiao-Yu ZHANG. Expression of laccase gene family in different development stages of Pleurotus ostreatus[J]. Mycosystema, 2015, 34(4): 712-716. DOI: 10.13346/j.mycosystema.150075 | |
| Authors: | Rui ZHUO Fu-Ying MA Shuai ZHOU Xiao-Yu ZHANG |
| Affiliation: | College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China |
| Abstract: | Laccases are involved in lignin degradation and mushroom development. In order to further determine the role of laccases in fruiting of Pleurotus ostreatus, expressions of 11 laccase genes and a small subunit of Lacc2 (sspoxa3) in different stages of fungal development were analyzed by real-time PCR. lacc6 and sspoxa3 are highly expressed throughout the growth and development stage. lacc12 is highly expressed during primordium differentiation and fruiting body formation, suggesting that lacc12 functions in fruiting. lacc4, lacc7 and lacc11 are highly expressed in primordium stage, indicating they are related with primordium differentiation. lacc2, lacc3 and lacc8 are relevant to fruiting body differentiation and maturation, with high expression during mature fruiting stage. |
| Keywords: | edible mushroom laccase primordium fruiting body real-time PCR |
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