WAVE2 deficiency reveals distinct roles in embryogenesis and Rac-mediated actin-based motility |
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Authors: | Yan Catherine Martinez-Quiles Narcisa Eden Sharon Shibata Tomoyuki Takeshima Fuminao Shinkura Reiko Fujiwara Yuko Bronson Roderick Snapper Scott B Kirschner Marc W Geha Raif Rosen Fred S Alt Frederick W |
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Institution: | Center for Blood Research, 200 Longwood Avenue, Boston, MA 02115, USA. |
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Abstract: | The Wiskott-Aldrich syndrome related protein WAVE2 is implicated in the regulation of actin-cytoskeletal reorganization downstream of the small Rho GTPase, Rac. We inactivated the WAVE2 gene by gene-targeted mutation to examine its role in murine development and in actin assembly. WAVE2-deficient embryos survived until approximately embryonic day 12.5 and displayed growth retardation and certain morphological defects, including malformations of the ventricles in the developing brain. WAVE2-deficient embryonic stem cells displayed normal proliferation, whereas WAVE2-deficient embryonic fibroblasts exhibited severe growth defects, as well as defective cell motility in response to PDGF, lamellipodium formation and Rac-mediated actin polymerization. These results imply a non-redundant role for WAVE2 in murine embryogenesis and a critical role for WAVE2 in actin-based processes downstream of Rac that are essential for cell movement. |
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