| C57BL/6J小鼠超数排卵的研究 |
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| 引用本文： | 戴丽军,黄月玲,叶炳飞.C57BL/6J小鼠超数排卵的研究[J].中国实验动物学报,2003,11(1):51-53. |
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| 作者姓名： | 戴丽军 黄月玲 叶炳飞 |
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| 作者单位： | 广州医学院实验动物研究中心,广州,510182 |
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| 基金项目： | 广东省医学科学技术研究基金资助项目 (A19992 2 0 ) |
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| 摘 要： | 目的 确定C57BL 6J小鼠超排的最佳激素剂量和最合适的注射间隔时间 ,提高超排率。方法 40只C57BL 6J雌鼠随机分为四组 ,分别用 5IU或 10IU的PMSG和HCG ,间隔 48h或 72h注射 ,比较排出卵母细胞的数量。结果 5IU +5IU剂量的PMSG和HCG、间隔 48h注射组超排效果最好 ;8～ 10周龄雌鼠较 6～ 8周龄雌鼠超排效果好。结论 C57BL 6J小鼠超排的最佳激素剂量为 5IUPMSG +5IUHCG ,最合适的注射间隔时间为 48h ,处于繁殖期的雌鼠超排效果好。
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| 关 键 词： | 小鼠 近交C57BL 超排卵 ACTH促性腺激素 受体 促性腺素 |
| 文章编号： | 1005-4847(2003)01-0051-03 |
| 修稿时间： | 2002年7月22日 |
Study of Superovulation in C57BL/6J Mise |
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| DAI Li-jun,HUANG Yue-ling,YEI Bing-fei.Study of Superovulation in C57BL/6J Mise[J].Acta Laboratorium Animalis Scientia Sinica,2003,11(1):51-53. |
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| Authors: | DAI Li-jun HUANG Yue-ling YEI Bing-fei |
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| Abstract: | Objective For the purpose of studing superovulation in C 57BL/6J mice. Method 5IU+5IU or 10IU+10IU PMSG and HCG were used to stimulate superovulation of C 57BL/6J mice. After PMSG given at 4:30 p.m. on the first day, HCG was injected on the third or fourth day (48 h or 72 h later). Ova were flushed out of the oviduct 16h after administration of HCG. The ova's amount of each group was compared. Result The superovulation in first group (5IU+5IU PMSG and HCG, 48 h) was best. The ova's amount of mice in 8-10 weeks by superovulation was more than that in 6-8 weeks. Conclusion In C 57BL/6J mice, the best dosage of hormone for superovulation is 5IU PMSG + 5IU HCG, and the best interval of time for the administration is 48h. |
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| Keywords: | Mice Inbred C57BL Superovulation ACTH Adrenocorticotropic Hormone Receptor Gonadotropin |
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