| 原核系统可溶性表达策略 |
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| 引用本文： | 刘爽,胡宝成. 原核系统可溶性表达策略[J]. 生物技术通讯, 2005, 16(2): 172-175 |
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| 作者姓名： | 刘爽 胡宝成 |
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| 作者单位： | 军事医学科学院,生物工程研究所,北京,100850 |
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| 摘 要： | 获得大量目的蛋白的最简单最经济的方法是利用原核表达系统表达外源基因.但由于原核系统的自身特点,使所表达的蛋白常常形成无活性的包涵体.多年来世界各国的研究为解决这一问题尝试了多种方法.本简单介绍原核表达系统的特点及提高蛋白可溶性表达的常用方法.
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| 关 键 词： | 大肠杆菌 可溶性表达 原核系统 蛋白质 包涵体 基因表达系统 |
| 文章编号： | 1009-0002(2005)02-0172-04 |
| 修稿时间： | 2004-09-20 |
Strategy of Protein Soluble Expression in Escherichia coli |
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| LIU Shuang,HU Bao-Cheng. Strategy of Protein Soluble Expression in Escherichia coli[J]. Letters in Biotechnology, 2005, 16(2): 172-175 |
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| Authors: | LIU Shuang HU Bao-Cheng |
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| Affiliation: | LIU Shuang,HU Bao-chengInstitute of Biotechnology,Academy of Military Medical Sciences,Beijing 100850,China |
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| Abstract: | The expression of heterologous genes in bacteria is the simplest and most economical means available for obtaining large amounts of desired proteins. As the prokaryocyte system's limitation, the expressed proteins often accumulate in insoluble and inactive form as inclusion body. Some methods have been tried for the problems. In this review, the character of the prokaryocytic expression system and the methods of improving soluble expression are described. |
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| Keywords: | Escherichia coli soluble expression |
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