Effects of inhibitors of polyamine biosynthesis and of polyamines on strawberry microcutting growth and development |
| |
Authors: | E Tarenghi M Carré J Martin-Tanguy |
| |
Institution: | (1) Station de Génétique d'Amélioration des Plantes, INRA, BV 1540, 21034 Dijon Cédex, France |
| |
Abstract: | The primary free polyamines identified during growth and development of strawberry (Fragaria × ananassa Duch.) microcuttings cultivated in vitro were putrescine, spermidine and spermine. Polyamine composition differed according to tissue and stages of development; putrescine was predominant in aerial green tissues and roots. -DL-difluoromethylarginine (DFMA), a specific and irreversible inhibitor of the putrescine-synthesizing enzyme, arginine decarboxylase (ADC), strongly inhibited growth and development. Application of agmatine or putrescine to the inhibited system resulted in a reversal of inhibition, indicating that polyamines are involved in regulating the growth and development of strawberry microcuttings. -DL-difluoromethylornithine (DFMO), a specific and irreversible inhibitor of putrescine biosynthesis by ornithine decarboxylase, promoted growth and development. We propose that ADC regulates putrescine biosynthesis during microcutting development. The application of exogenous polyamines (agmatine, putrescine, spermidine) stimulated development and growth of microcuttings, suggesting that the endogenous concentrations of these polyamines can be growth limiting.Abbreviations ADC
arginine decarboxylase
- ODC
ornithine decarboxylase
- DFMA
-difluoromethylarginine
- DFMO
-difluoromethylornithine
- Put
putrescine
- Spd
spermidine
- Sp
spermine
- DW
dry weight
- PA
polyamine
- PPF
photosynthetic photon flux |
| |
Keywords: | Arginine decarboxylase Fragaria × ananassa Duch micropropagation in vitro ornithine decarboxylase polyamines polyamine inhibitors |
本文献已被 SpringerLink 等数据库收录! |
|