DNA aqueous solution used for dialytical removal and enrichment of dioxin derivatives |
| |
Authors: | Liu Xiang Dong Murayama Yoko Yamada Masanori Nomizu Motoyoshi Matsunaga Masaji Nishi Norio |
| |
Institution: | a Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060-0810, Japan b Nissei Bio Co., Ltd, Tokyo 130-0026, Japan |
| |
Abstract: | In the present study, a dialytic method that uses a DNA aqueous solution to remove and enrich dioxins from polluted water was proposed. Circular dichroism (CD) and fluorescent spectra indicated that dibenzo-p-dioxin (DD), dibenzofuran (DF) and biphenyl (BP), which are dioxin derivatives, form complexes with DNA. Their experimental dialytic sorption coefficients were measured by quantifying the concentrations of DD, DF, and BP in aqueous solutions before and after dialysis of the DNA solution, and the values were 2.1×105, 1.3×105, and 1.5×107, respectively. As a simulated water treatment model, DNA solution was dialyzed in an aqueous mixture of DD, DF, and BP for 96 h, the HPLC studies showed that the dioxin derivatives have been concentrated in the DNA solution about 200 times. The dialyzed DNA solution was reusable by an extraction with hexane. |
| |
Keywords: | DNA Dialysis Dioxins |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|