Desulfurization of light gas oil by a novel recombinant strain from Pseudomonas
aeruginosa |
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Authors: | Kimiko Watanabe Ken-ichi Noda Yoshinori Ohta Kenji Maruhashi |
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Institution: | (1) Bio-Refining Process Laboratory, Technical Cooperation Department, Japan Cooperation Center, Petroleum, 1900 Sodeshi-cho, Shimizu-shi, Shizuoka, 424-0037, Japan |
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Abstract: | The dsz desulfurization gene cluster from Rhodococcus erythropolis KA2-5-1 was transferred into the chromosomes of Pseudomonas aeruginosa NCIMB 9571 by using a transposon vector. Resting cells of the recombinant strain, PAR41, desulfurized 63 mg sulfur l–1 of light gas oil (LGO) containing 360 mg S l–1. The desulfurization activity for LGO by the resting cells of strain PAR41 grown with n-tetradecane (50% v/v) was much higher (10 18-fold) than in glucose-grown cells. P. aeruginosa NCIMB 9571 is able to take up water-insoluble compounds from an oil phase which is enhanced by n-alkane. |
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Keywords: | n-alkane desulfurization LGO Pseudomonas aeruginosa uptake |
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