| 苦豆子的组织培养及植株再生的研究 |
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| 引用本文： | 曹有龙,李晓莺,罗 青,贝盏临.苦豆子的组织培养及植株再生的研究[J].广西植物,2010,30(1):102-105. |
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| 作者姓名： | 曹有龙 李晓莺 罗 青 贝盏临 |
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| 作者单位： | 宁夏农林科学院,枸杞工程技术研究中心,银川,750002 |
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| 基金项目： | 宁夏回族自治区科技攻关项目(2001-015-04)~~ |
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| 摘 要： | 用4种诱导培养基P1(MS+2,4-D0.5mg/L)、P2(MS+6-BA0.1mg/L+NAA0.5mg/L)、P3(MS+6-BA0.5mg/L+NAA0.5mg/L)、P4(MS+NAA1.0mg/L+KT0.5mg/L),3种分化培养基(MS+6-BA1mg/L;MS+6-BA0.5mg/L;MS+6-BA1mg/L+NAA0.2mg/L)和4种生根培养基(MS;MS+IBA1mg/L;MS+IBA1mg/L+IAA0.5mg/L;1/2MS+IAA0.2mg/L)对苦豆子愈伤组织进行诱导和植株再生,研究影响苦豆子组织培养的因素,结果表明愈伤组织的诱导频率主要依靠激素的种类和浓度,培养基中加入0.2～2mg/L的2,4-D有利于苦豆子愈伤组织生长,但使褐化发生时间提前;培养基中加入活性炭对苦豆子愈伤组织褐化有明显的抑制作用;加入IAA对苦豆子根的分化是必需的。
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| 关 键 词： | 苦豆子 组织培养 植株再生 |
Tissue culture and plant regeneration
of Sophora alopecuroides |
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| CAO You-Long,LI Xiao-Ying,LUO Qing,BEI Zhan-Lin.Tissue culture and plant regeneration
of Sophora alopecuroides[J].Guihaia,2010,30(1):102-105. |
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| Authors: | CAO You-Long LI Xiao-Ying LUO Qing BEI Zhan-Lin |
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| Institution: | Engineering and Technology Research Center of Wolfberry;Ningxia Academy of Agricultural and Forestry Sciences;Yinchuan 750002;China |
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| Abstract: | In order to study the factors that influence the tissue culture of Sophora alopecuroikes,four callus-inducing media,i.e.P1(MS+2,4-D 0.5 mg/L),P2(MS+6-BA 0.1 mg/L+NAA 0.5 mg/L),P3(MS+6-BA 0.5 mg/L+NAA 0.5 mg/L),P4(MS+NAA 1.0 mg/L+KT 0.5 mg/L),three different media(MS+6-BA 1 mg/L;MS+6-BA 0.5 mg/L;MS+6-BA 1 mg/L+NAA 0.2 mg/L),and four root-inducing media(MS;MS+IBA 1 mg/L;MS+IBA 1 mg/L+IAA 0.5 mg/L;1/2 MS+IAA 0.2 mg/L)were applied. The results showed that the induction frequency of calluses highly depended on t... |
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| Keywords: | Sophora alopecuroides tissue culture plant regeneration |
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