Addressing the overlap problem in the quantitative analysis of two dimensional NMR spectra: Application to 15N relaxation measurements |
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| Authors: | Vitali Tugarinov Wing-Yiu Choy Eriks Kupče Lewis E. Kay |
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| Affiliation: | (1) Protein Engineering Network Centres of Excellence and the Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, M5S lA8 Toronto, Ontario, Canada;(2) Varian Ltd., Eynsham, Oxford, UK |
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| Abstract: | A quantitative analysis of 2D 1H-15N spectra is often complicated by resonance overlap. Here a simple method is presented for resolving overlapped correlations by recording 2D projection planes from HNCO data sets. Applications are presented involving the measurement of 15N T1 relaxation rates in a high molecular weight protein, malate synthase G, and in a system that exchanges between folded and unfolded states, the drkN SH3 domain. By supplementing relaxation data recorded in the conventional way as a series of 2D 1H-15N data sets with a series of a pair of projection planes the number of dynamics probes is increased significantly for both systems studied. |
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| Keywords: | HNCO 15N relaxation plane projections resonance overlap |
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