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酶联免疫吸附检测法的应用研究进展
引用本文:伏旭,李培武,贺莉,尹稳,傅仲学.酶联免疫吸附检测法的应用研究进展[J].氨基酸和生物资源,2012,34(3):41-44.
作者姓名:伏旭  李培武  贺莉  尹稳  傅仲学
作者单位:1. 兰州大学第二医院急救中心急诊研究室,甘肃,兰州,730030;2. 重庆医科大学附属第一医院普外科,重庆,400030;3. 兰州大学第二医院,甘肃,兰州,730030
基金项目:甘肃省技术研究与开发专项计划项目(0709TCYA062)
摘    要:酶联免疫吸附检测法(ELISA)由于具有灵敏、特异、简单、快速及易于自动化操作等优点而得到了快速发展,并且被广泛应用于多个领域.本文对ELISA检测法应用现状及优缺点进行综述,为今后ELISA检测法的更为广阔的应用与发展提供参考.

关 键 词:酶联免疫吸附检测法  抗原  抗体  应用现状

Progress on Application of Enzyme-Linked Immunosorbent Assay
FU Xu,LI Peiwu,HE Li,YIN Wen,FU Zhongxue.Progress on Application of Enzyme-Linked Immunosorbent Assay[J].Amino Acids & Biotic Resources,2012,34(3):41-44.
Authors:FU Xu  LI Peiwu  HE Li  YIN Wen  FU Zhongxue
Institution:1.Research Division of Emergency Medicine,Emergency Center,Second Hospital of Lanzhou University,Lanzhou 730030,Gansu,China; 2.Department of General Surgeon,First Affiliated Hospital of Chongqing Medical University,Chongqing 400030,China; 3.Second Hospital of Lanzhou University,Lanzhou 730030,Gansu,China)
Abstract:The Enzyme-Linked Immunosorbent Assay(ELISA) has been rapidly developed and widely used in various fields because of its sensitivity,specificity,simpleness and ease for automattic operation.This article mainly summarizes the application of ELISA methodology and its advantages and existing problems,to provide a reference for the broader application and development of ELISA in the future.
Keywords:ELISA  antigen  antibody  application status
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