Endothelial specific granules in the umbilical veins of the postnatal rabbit |
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Authors: | Fujimoto Sunao Yamamoto Koji Arashidani Keiichi Hayabuchi Ichiro Yoshizuka Mitsuaki Nomiyama Tomohiko |
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Institution: | (1) Department of Anatomy, School of Medicine, University of Occupational and Environmental Health, Kitakyushu;(2) Department of Dermatology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu;(3) Section of Occupational Hygiene, School of Medicine, University of Occupational and Environmental Health, Kitakyushu;(4) Department of Anatomy, Kurume University School of Medicine, Kurume, Japan;(5) Department of Anatomy, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka Yahata Nishi Ku, 807 Kitakyushu, Japan |
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Abstract: | Summary A remarkable increase in number of endothelial specific granules was observed in the rabbit umbilical veins between 2 and 5 days after birth. Electron microscopy indicated that the granules were segregated in the Golgi complex of the endothelial cells and released into the vascular lumen during the postnatal obliteration stage of this vessel.Incubation of the postnatal vessels in Ringer solution containing a histamine releasing compound induced remarkable morphological alterations of these cytoplasmic components; a reduction of their osmiophilia, swelling with a widened space separating the granular matrix from the limiting membrane, fusion to each other and expulsion of their contents into the vascular lumen, as in mast cell degranulation by this drug, were noted.High-performance liquid chromatography of the homogenized vessels demonstrated appreciable concentrations of histamine in the postnatal samples. There was a correlation between the histamine concentration and the quantity of granules in the respective postnatal samples.The present study strongly suggests that the granules are reservoirs of histamine and have an important role in the obliteration of this vessel.This work was supported in part by Grant in Aid for Scientific Research (# 448087) to S. Fujimoto from the Ministry of Education of Japan |
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Keywords: | Endothelial specific granules Rabbit umbilical vein Compound 48/80 High-performance liquid chromatography Histamine |
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