Type 2 diabetes mellitus in relation to global LINE-1 DNA methylation in peripheral blood: A cohort study |
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Authors: | Gracia María Martín-Nú?ez Elehazara Rubio-Martín Rebeca Cabrera-Mulero Gemma Rojo-Martínez Gabriel Olveira Sergio Valdés Federico Soriguer Luis Casta?o Sonsoles Morcillo |
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Institution: | 1.UGCI de Endocrinología y Nutrición; Instituto de Investigación Biomédica de Málaga (IBIMA); Hospital Regional Universitario; Malaga, Spain;2.University of Málaga (UMA); Malaga, Spain;3.CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM); Spain;4.Endocrinology and Diabetes Research Group; Hospital de Cruces and University of the Basque Country UPV-EHU; Barakaldo, Spain |
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Abstract: | In the last years, epigenetic processes have emerged as a promising area of complex diseases research. DNA methylation measured in Long Interspersed Nucleotide Element 1 (LINE-1) sequences has been considered a surrogate marker for global genome methylation. New findings have suggested the potential involvement of epigenetic mechanisms in Type 2 diabetes (T2DM) as a crucial interface between the effects of genetic predisposition and environmental influences. Our study evaluated whether global DNA methylation predicted increased risk from T2DM or other carbohydrate metabolism disorders in a cohort study. We used a prospective cohort intervention study and a control group. We collected phenotypic, anthropometric, biochemical, and nutritional information from all subjects. Global LINE-1 DNA methylation was quantified by pyrosequencing technology. Subjects that did not improve their carbohydrate metabolism status showed lower levels of global LINE-1 DNA methylation (63.9 ± 1.7 vs. 64.7 ± 2.4) and they practiced less intense physical activity (5.8% vs. 21.5%). Logistic regression analyses showed a significant association between LINE-1 DNA methylation and metabolic status after adjustment for sex, age, BMI, and physical activity. Our study showed that lower LINE-1 DNA methylation levels were associated with a higher risk metabolic status worsening, independent of other classic risk factors. This finding highlights the potential role for epigenetic biomarkers as predictors of T2DM risk or other related metabolic disorders. |
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Keywords: | cohort study diabetes DNA methylation LINE-1 peripheral blood cell |
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