哈尼梯田稻-渔共作模式下杂交黄颡鱼肠道微生物研究

为了探究哈尼梯田稻-鱼共作综合种养模式(稻渔组, DY组)和传统池塘养殖模式(池塘组, CT组)下杂交黄颡鱼(Tachysurus fulvidraco♀×Pseudobagrus vachellii♂)肠道微生物结构变化,试验采用16S rDNA测序技术对不同养殖模式下黄颡鱼肠道微生物进行分析。测序结果显示, CT组和DY组优势菌门均为变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)和梭杆菌门(Fusobacteria)。和CT组相比,在门水平上DY组厚壁菌门和拟杆菌门相对丰度显著上升,而变形菌门相对丰度显著下降。在属水平上DY组梭状芽孢杆菌属、Romboutsia属、Paludibacter属、Epulopiscium属和拟杆菌属相对丰度显著上升,而邻单胞菌属丰度显著下降。不同的养殖模式没有显著影响黄颡鱼肠道微生物的丰富度(Richness),但DY组拥有更高的微生物均匀度(Evenness)。BugBase表型预测结果如下, CT组革兰氏阴性菌,兼性厌氧菌丰度更高, DY组则革兰氏阳性菌,厌氧菌丰度更高。同时DY组...

MICROBIAL COMMUNITY STRUCTURE OF HYBRID YELLOW CATFISH IN RICE-FISH CO-CULTURE SYSTEM IN HANI TERRACE

Yellow catfish is a high-quality and popular fish that is widely grown in freshwater farms. The rice-fish co-culture systems in Hani Terrace is a new agricultural model. To explore the differences of intestinal microbiota of hybrid yellow catfish (Tachysurus fulvidraco♀×Pseudobagrus vachellii♂) between rice-fish co-culture system (DY group) and traditional pond system (CT group), we conducted feeding experiment in terraces and ponds at an altitude of 634 meters in Honghe Hani Autonomous Prefecture of Yunnan Province. The 16S rRNA gene profiling analysis showed that the dominant flora of yellow catfish were Proteobacteria, Firmicutes, Bacteroidetes and Fusobacteria. Different culture patterns did not change the dominant microflora of yellow catfish, but affected the relative abundance of dominant flora. Compared with the CT group, the DY group had more abundance of Firmicutes and Bacteroidetes and less Proteobacteria at phylum level. At genus level, compared with DY group, the CT group had significantly increased abundance of Plesiomonas and significantly decreased abundance of genera Clostridium_sensu_stricto_1, Romboutsia, Paludibacter, Epulopiscium, and Bacteroides. Different culture patterns did not significantly affect the intestinal microbial richness of yellow catfish, but DY group had higher microbial evenness. Moreover, gram_negative and facultatively anaerobic microorganisms were enriched in CT group, and gram_positive and anaerobic microorganisms were enriched in DY group. In addition, gene related to biofilm forming and potential_pathogenicity were depleted in the DY group. These results indicate that DY group had higher intestinal microbial diversity, better stability and stronger resistance to diseases. The rice-fish co-culture system can provide a better living environment for yellow catfish.