scDNA-DNA hybridization studies in Pacific and Caribbean isolates ofDictyosphaeria cavernosa (Chlorophyta) indicate a long divergence |
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| Authors: | J L Olsen W T Stam P V M Bot C van den Hoek |
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| Institution: | (1) Department of Marine Biology, Biology Centre, University of Groningen, P. O. Box 14, NL-9750 AA Haren (Gn), The Netherlands;(2) Present address: Department of Botany, LSB-2017, University of California, 94720 Berkeley, CA, USA |
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| Abstract: | Genomic size and complexity were calculated for the pantropical chlorophyte,Dictyosphaeria cavernosa (Cladophorales). Genome characterization of the Hawaiian material by means of DNA renaturation studies showed highly repetitive
(31.3%), middle repetitive (42.7%), and single-copy (25.8%) fractions; and an estimated haploid genome size of 1.79 pg DNA.
A G:C content of 43% was calculated from a melting curve of DNA. Pacific and Caribbean isolates of this species were compared
using single-copy nuclear DNA-DNA hybridization. Results show a relatively low thermal stability of the hybridized DNA (Δ
Tm(e)=10°C) which suggests that these Pacific and Caribbean lineages may have been separated for up to 55 Ma. |
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