Hydrogen sulphide production by Zymomonas and its elimination in a mutant strain |
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Authors: | Amanda E Goodman Peter L Rogers |
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Institution: | School of Biotechnology, University of New South Wales, Kensington, Sydney, NSW 2033, Australia |
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Abstract: | Strains of Zymomonas mobilis grown in media containing either glucose or sucrose were assessed for the production of hydrogen sulphide (H2S). In a liquid medium with low glucose concentration (20 g l?1) only a proportion of the strains tested formed H2S, but in medium containing a higher glucose concentration (100 g l?1) all the strains tested produced H2S. Four Z. mobilis strains were assayed quantitatively for H2S production and strain ZM4 was found to produce the most H2S in glucose medium. The amount of yeast extract and glucose, and the type of sugar used in the medium affected the amount of H2S formed by strain ZM4. A mutant, designated ZM4701, of strain ZM4 was isolated which did not produce any detectable H2S in liquid medium containing yeast extract plus either glucose or sucrose. The nutritional requirements of ZM4701 were investigated. |
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Keywords: | mutation high glucose concentration |
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