Addition of Polybrene improves stability of organophosphate hydrolase immobilized in poly(vinyl alcohol) cryogel carrier |
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| Authors: | Efremenko Elena N Lozinsky Vladimir I Sergeeva Viktoriya S Plieva Fatima M Makhlis Tatiana A Kazankov Grigori M Gladilin Alexander K Varfolomeyev Sergei D |
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| Affiliation: | Chemical Enzymology Division of Chemical Department, M.V. Lomonosov Moscow State University, Moscow, 119899, Russia. efremenko@enzyme.chem.msu.ru |
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| Abstract: | Organophosphate hydrolase, covalently attached to the beads of poly(vinyl alcohol) cryogel in the presence of Polybrene, was fivefold more stable in 15% (v/v) ethanol solution than the free enzyme. Immobilized biocatalyst, prepared with an addition of Polybrene, retained a half of its initial activity in 50% (v/v) aqueous ethanol solution, 90% of activity during 10 working cycles of Paraoxon hydrolysis and 85% of activity after storage in the 50 mM CHES buffer (pH 9.0) at room temperature for 2 months. |
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