经穴注射BMSCs联合中药对大鼠血清VEGF、G-CSF、SDF-1浓度的影响

目的：前期基础实验发现经穴注射骨髓间充质干细胞联合益气活血中药对大鼠缺血后肢骨骼肌血管密度以及后肢血流恢复具有明显促进作用,为进一步明确其机制,本研究着重经穴注射骨髓间充质干细胞（BMSCs）联合益气活血方对大鼠血清中血管内皮细胞生长因子（VGEF）,粒细胞集落刺激因子（G-CSF）,基质细胞衍生因子-1（SDF-1）浓度的影响,从而为临床中医血管外科防治后肢动脉缺血性疾病提供新的思路和方法。方法：25只大鼠随机分为空白对照组（CG）、下肢缺血模型组（IG）、益气活血方缺血组（HG）、经穴注射BMSCs缺血组（BG）,经穴注射BMSCs＋益气活血方缺血组（BHG）,每组5只,在给予相应干预措施后,于7天后取血清,用酶联免疫吸附法（ELISA）测定大鼠血清中VEGF、G-CSF、SDF-1质量浓度。结果：1与CG比较,IG、HG、BG、BHG大鼠血清中VEGF、SDF-1、G-CSF浓度显著升高（P〈0.01）；2与IG比较,HG、BG、BHG组大鼠血清中VEGF,G-CSF浓度显著升高（P〈0.01）,BG、BHG大鼠血清中SDF-1浓度显著升高（P〈0.01）；3与HG比较,BG、BHG大鼠血清中VEGF,SDF-1,G-CSF浓度显著升高（P〈0.01）；4与BG比较,BHG大鼠血清中VEGF,SDF-1,G-CSF浓度显著升高（P〈0.01）,以上差异均有统计学意义。结论：经穴注射BMSCs联合益气活血中药可大幅提升下肢缺血模型大鼠血清中VEGF、G-CSF、SDF-1质量浓度,为临床中医血管外科防治后肢动脉缺血性疾病提供了新的思路和方法。

Effect of Concentration on VEGF,G-CSF,SDF-1 Stimulated by Acupoint Injection BMSCs Combined Chinese Herb in Rat Serum

Objective： It was found in our early basic experiment that acupoint injection of bone marrow mesenchymal stem cells combined Qi-tonifying and Blood-activating Chinese herb have obvious effect of promoting the vascular density and restoring the blood flow to skeletal muscle of SD rat with hind limbs ischemic. In order to clarify the further mechanism, this study was designed to investigate the concentration of vascular endothelial cell growth factor（VGEF）, granulocyte colony stimulating factor（G-CSF）, stromal cell derived factor 1（SDF-1） stimulated by acupoint injection of bone marrow mesenchymal stem cells（BMSCs） transplantation combined Chinese Herb in rat serum, so as to provide new methods for prevention and cure arterial ischemic disease in vascular surgery of traditional Chinese medicine. Methods： 25 rats were randomly divided into control group（CG）, lower limb ischemia model group（IG）,Herb group（HG）, Acupoint injection of BMSCs ischemia group（BG）, Acupoint injection of BMSCs ＋Herb ischemia group（BHG）, and given 7 days corresponding intervention measures. Then we measured the concentration of VEGF, G-CSF, SDF-1in rat serum by Enzyme Linked Immunosorbent Assay（ELISA）. Results： 1 Compared with CG, concentration of VEGF, G-CSF, and SDF-1 increased significantly（P〈 0.01） in IG, HG, BG, BHG; 2Compared with IG, concentration of VEGF and G-CSF increased significantly（P 〈 0.01）in HG, BG, BHG, while SDF- 1 increased（P 〈 0.01） in BG, BHG; 3Compared with HG, concentration of VEGF, G-CSF, and SDF-1increased significantly（P 〈 0.01） in BG, BHG; 4 Compared with BG, concentration of VEGF, G-CSF, and SDF-1 increased significantly（P 0.01） in BHG. The above all differences were statistically significant. Conclusion： Acupoint injection of BMSCs combined Qi-tonifying and Blood-activating Chinese herb can be sharply raised the concentration of VEGF, G-CSF and SDF-1 in the lower limb ischemia rat model, which provide new methods for prevention and cure