Trishanku, a novel regulator of cell-type stability and morphogenesis in Dictyostelium discoideum |
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Authors: | Jaiswal Jyoti K Mujumdar Nameeta Macwilliams Harry K Nanjundiah Vidyanand |
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Institution: | Department of Molecular Reproduction Development and Genetics, Indian Institute of Science, Bangalore 560012, India. jaiswaj@rockefeller.edu |
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Abstract: | We have identified a novel gene, trishanku (triA), by random insertional mutagenesis of Dictyostelium discoideum. TriA is a Broad complex Tramtrack bric-a-brac domain-containing protein that is expressed strongly during the late G2 phase of cell cycle and in presumptive spore (prespore (psp)) cells. Disrupting triA destabilizes cell fate and reduces aggregate size; the fruiting body has a thick stalk, a lowered spore: stalk ratio, a sub-terminal spore mass and small, rounded spores. These changes revert when the wild-type triA gene is re-expressed under a constitutive or a psp-specific promoter. By using short- and long-lived reporter proteins, we show that in triA(-) slugs the prestalk (pst)/psp proportion is normal, but that there is inappropriate transdifferentiation between the two cell types. During culmination, regardless of their current fate, all cells with a history of pst gene expression contribute to the stalk, which could account for the altered cell-type proportion in the mutant. |
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Keywords: | REMI differentiation pattern formation BTB domain trishanku β glucoronidase labile β galactosidase |
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