Viral diagnosis in Indian livestock using customized microarray chips |
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| Authors: | Brijesh S Yadav Mayank Pokhriyal Barkha Ratta Ajay Kumar Meeta Saxena Bhaskar Sharma |
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| Affiliation: | Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, India - 243122 |
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| Abstract: | Viral diagnosis in Indian livestock using customized microarray chips is gaining momentum in recent years. Hence, it is possible to
design customized microarray chip for viruses infecting livestock in India. Customized microarray chips identified Bovine herpes
virus-1 (BHV-1), Canine Adeno Virus-1 (CAV-1), and Canine Parvo Virus-2 (CPV-2) in clinical samples. Microarray identified specific
probes were further confirmed using RT-PCR in all clinical and known samples. Therefore, the application of microarray chips
during viral disease outbreaks in Indian livestock is possible where conventional methods are unsuitable. It should be noted that
customized application requires a detailed cost efficiency calculation. |
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| Keywords: | DNA microarray viral diagnosis livestock RT-PCR |
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