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Ensuring oxygenation of carrot cell cultures in a Couette viscometer during investigation of shear effects |
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| Authors: | Victor Wong David Williams Christopher B. Colby |
| Affiliation: | (1) Department of Chemical Engineering, Adelaide University, Adelaide, SA, 5005, Australia;(2) Bioprocessing Technology Centre, National University of Singapore, 10 Kent Ridge Crescent, Singapore, 119260 |
| Abstract: | Mathematical simulation and experimental measurement of dissolved O2 were performed for extended (up to 8 h) shear testing of Daucus carota (carrot) cell cultures in a conventional Couette viscometer (0.625 mm annulus). The results suggest O2 depletion below critical levels for cell growth may occur. A novel design modification incorporating an O2-permeable silicone-layer spun cast on a porous ceramic bowl was devised. It significantly improved oxygenation of the cell cultures, keeping dissolved O2 near saturation. |
| Keywords: | Couette viscometer mathematical simulation oxygen depletion plant cell culture shear sensitivity |
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