痢疾杆菌侵袭HeLa细胞基因表达谱的研究

采用cDNA微阵列技术检测了HeLa细胞被痢疾杆菌侵袭1h和3h后的基因表达变化,共发现2倍以上差异表达基因752个,上调基因有509个,下调基因有306个,并初步推测HeLa细胞通过激活某些信号通路,诱导表达多个基因,产生整体的细胞效应,以对抗痢疾杆菌的侵袭。对显著差异表达的两个基因TNFR 1B和ERBB2,在痢疾杆菌侵袭HeLa细胞1h和3h后的表达量经荧光实时定量PCR验证,确定这两个基因的确在痢疾杆菌侵袭期间高表达,它们在细胞对痢疾杆菌2457T侵袭反应中起重要的作用。这些结果促进了对痢疾杆菌分子致病机理的认识,也为形成预防和治疗痢疾的策略提供了理论基础。

Expression profile analysis of host HeLa cells invasived by Shigella flexneri 2a

The changes of genes expression in HeLa cell during the invasion with Shigella species for 1h and 3h were analyzed by cDNA microarrays. The data showed that the expression levels of 752 genes were altered twice or greater as compared with the control 509 of them were up-regulated,and 306 were down-regulated. It was supposed that some signal pathways in HeLa cell were activated,then many genes were induced,and at last comprehensive cell responses were produced,so that HeLa cell could prevent against Shigella species infection. Two striking difference cDNA fragments TNFR 1B and ERBB2,which were up-regulated in the host epithelial cell during Shigella infection,analyzed expression by real time RT-PCR as determined by cDNA arrays. We suggested they play important roles in response to the invasive S. flexneri 2457T. Based on the results of gene expression alterations,the molecular pathogenic mechanism of Shigella species could be greatly and deeply understood,and the strategy for prevention against and treatment for shigellosis would be formed.