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| CRF01-AE亚型人类免疫缺陷病毒的基因序列特征研究 | |
| 引用本文： | 关琪,王素芬,张卓然,王宝江.CRF01-AE亚型人类免疫缺陷病毒的基因序列特征研究[J].微生物学杂志,2005,25(5):33-35. |
| 作者姓名： | 关琪 王素芬 张卓然 王宝江 |
| 作者单位： | 1. 沈阳医学院,奉天医院检验科,辽宁,沈阳,110024 2. 辽宁大学,法学院,辽宁,沈阳,110036 3. 大连医科大学,微生物学教研室,辽宁,大连,116027 4. 沈阳医学院,奉天医院药剂科,辽宁,沈阳,110024 |
| 摘 要： | 从确诊的HIV-1感染者的全血样本中提取基因组DNA,经套式聚合酶链反应(PCR)扩增其gag蛋白P17/P24交界区基因片断后,将扩增产物进行纯化和测序,分析其氨基酸序列。进而了解所检出的病毒基因变异和分子流行病学特征。结果发现,HIV-1 CRF01-AE亚型病毒分别与3株不同来源的国际参考毒株具有紧密的亲缘关系,表明这些毒株可能分别由不同的传播路线进入我国大陆境内。 |
| 关 键 词： | 人类免疫缺陷病毒1型 CRF01-AE亚型 序列分析 |
| 文章编号： | 1005-7021(2005)05-0033-03 |
| 修稿时间： | 2005年6月6日 |
Gene Sequence Characteristics of HIV-1 CRF01-AE Subtype Viruses |
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| GUAN Qi,WANG Su-fen,ZHANG Zuo-ran,WANG Bao-jiang.Gene Sequence Characteristics of HIV-1 CRF01-AE Subtype Viruses[J].Journal of Microbiology,2005,25(5):33-35. | |
| Authors: | GUAN Qi WANG Su-fen ZHANG Zuo-ran WANG Bao-jiang |
| Institution: | GUAN Qi~1,WANG Su-fen~2,ZHANG Zuo-ran~3,WANG Bao-jiang~4 |
| Abstract: | Genomic DNA was extracted from whole blood sample from HIV-1 diagnosed patient. Nested PCR were carried out and PCR products of gag P17/P24 gene were purified and used directly for sequencing.Sequence analysis of HIV-1 CRF01-AE subtype viruses was performed,and the characteristics of genetic variation of HIV-1 CRF01AE subtype viruses and molecular epidemiology were studied.The experiment showed that there were close relations between HIV-1 CRF01-AE subtype viruses and three different reference sequences of same subtype respectively.The results suggested that HIV-1 CRF01-AE subtype viruses in the experiment might be transferred into China by different transmit routes. |
| Keywords: | HIV-1 CRF01-AE subtype sequence analysis |
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