| 胰岛素蛋白质工程：高活性［B10Asp］人胰岛素 |
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| 引用本文： | 唐月华,刘卫东,刘滨,徐明华,冯佑民.胰岛素蛋白质工程：高活性［B10Asp］人胰岛素[J].中国生物化学与分子生物学报,1996,12(5):511-515. |
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| 作者姓名： | 唐月华 刘卫东 刘滨 徐明华 冯佑民 |
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| 作者单位： | 中国科学院上海生物化学研究所，分子生物学国家重点实验室 |
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| 摘 要： | 用基因定位突变方法将胰岛素Ｂ链第１０位的Ｈｉｓ变为Ａｓｐ，获得高活力胰岛素──［Ｂ１０Ａｓｐ］人胰岛素。其受体结合能力和离体生物活力分别为猪胰岛素的２６２％和２３５％；体内生物活力也明显高于猪胰岛素；它的促细胞生长能力为猪胰岛素的１７４％。
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| 关 键 词： | ［B10Asp］人胰岛素 蛋白质工程 高活力胰岛素 胰岛素促生长活性 |
| 收稿时间： | 1996-10-20 |
Protein Engineering of Insulin: a High Potential[B10Asp] Human Insulin |
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| Tang Yue-Hua,Liu Wei-Dong,Liu Bin,Xu Ming-Hua,Feng You-Min.Protein Engineering of Insulin: a High Potential[B10Asp] Human Insulin[J].Chinese Journal of Biochemistry and Molecular Biology,1996,12(5):511-515. |
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| Authors: | Tang Yue-Hua Liu Wei-Dong Liu Bin Xu Ming-Hua Feng You-Min |
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| Institution: | (Stare Key Laboratory of Molecular Biology,Shanghai Institute of Biochemistry, Academia Sinica,Shanghai 20003 1 |
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| Abstract: | B10His of insulin B chain was substituted with Asp by the means of site-directed mutagenesis.A high potent human insulin──B10Asp] insulin was obtained. Its binding capacity to the insulin receptor and in vitro biological activity are 262% and 235% respectivaly as those of porcine insulin;the in vivo biological activity of B10Aspj] human insulin is also higher than that of porcine insulin. The cell growth promoting activity of B10Asp] human insulin is 174% as that of porcine insulin. |
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| Keywords: | B10Asp] human insulin Protein engineering High potent insulin Growth promoting activity of insulin |
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