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The xylanase introns from Cryptococcus albidus are accurately spliced in transgenic tobacco plants
Authors:Jean- -François Laliberté  Olivier Nicolas  Serge Durand  Rolf Morosoli
Affiliation:(1) Institut Armand-Frappier, 531 Boulevard des Prairies, H7N 4Z3 Ville de Laval, Québec, Canada
Abstract:The xylanase gene from Cryptococcus albidus contains seven introns. Genomic and cDNA clones under the control of the CaMV 35S promoter were transferred into tobacco plants using Agrobacterium-mediated cell transformation. The genes were transcribed and the mRNAs were amplified by the polymerase chain reaction using primers on each side of the intron region. About 90% of the amplification products from plants transformed with the genomic clone corresponded to the size of the pre-mRNA (1.2 kb) and 10% represented the spliced product (0.85 kb). The 0.85 kb fragment was cloned and sequenced and the result indicated that the introns from the xylanase gene were accurately spliced by the plant cells.
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