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Expression of the Tick-borne Encephalitis Virus NS1 Gene in Gram-Negative Bacteria from the Mouse Nasopharynx |
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| Authors: | Morozova O V Maksimova T G Popova R V Martsinkevich O N Bakhvalova V N |
| Institution: | (1) Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Novosibirsk, 630090, Russia;(2) Novosibirsk State University, Novosibirsk, 630090, Russia;(3) Institute of Systematics and Ecology of Animals, Siberian Division, Russian Academy of Sciences, Novosibirsk, 630091, Russia |
| Abstract: | Bacteria were isolated from the nasopharynx of BALB/c mice and electroporated with pUR290(NS1)2 containing two copies of tick-borne encephalitis virus (TBEV, strain Sofjin) NS1 under the control of the lac promoter. The plasmid persisted in transformants for at least ten passages. The NS1 gene expression was detected in Gram-negative enterobacteria by immunoblotting with monoclonal antibodies against the TBEV nonstructural glycoprotein NS1. Recombinant NS1 was detected in bacterial cells and in the culture medium. Intranasal immunization with recombinant bacteria caused antibodies against NS1 to appear in the serum of BALB/c mice. However, the humoral immune response to NS1 failed to protect mice from a TBEV challenge. |
| Keywords: | tick-borne encephalitis virus nonstructural glycoprotein 1 recombinant bacteria electroporation mucosal immunization |
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