| 弗氏志贺菌毒力大质粒导入大肠杆菌MG1655 |
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| 引用本文： | 徐慧杰,朱力,胡威,冯尔玲,郑涛,王恒樑.弗氏志贺菌毒力大质粒导入大肠杆菌MG1655[J].生物技术通讯,2012,23(1):42-45. |
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| 作者姓名： | 徐慧杰 朱力 胡威 冯尔玲 郑涛 王恒樑 |
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| 作者单位： | 军事医学科学院生物工程研究所,北京,100071 |
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| 摘 要： | 目的:将弗氏2a志贺菌2457T的毒力大质粒pSF导入大肠杆菌MG1655。方法:通过诱动转移技术,将弗氏2a志贺菌2457T的毒力大质粒导入大肠杆菌MG1655。结果:构建了MG1655/pSF:pXL275-virG的毒力大质粒导入突变株,双向电泳初步比较分析表明在重组MG1655中有志贺菌毒力的表达。结论:成功地将弗氏2a志贺菌2457T毒力大质粒pSF导入了大肠杆菌MG1655。
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| 关 键 词： | 弗氏志贺菌 毒力大质粒 诱动转移 |
Conjugation of the Virulent Plasmid of Shigella flexneri into Escherichia coli MG1655 |
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| XU Hui-Jie
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ZHU Li
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HU Wei
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FENG Er-Ling
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ZHEGN Tao
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WANG Heng-Liang.Conjugation of the Virulent Plasmid of Shigella flexneri into Escherichia coli MG1655[J].Letters in Biotechnology,2012,23(1):42-45. |
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| Authors: | XU Hui-Jie ZHU Li HU Wei FENG Er-Ling ZHEGN Tao WANG Heng-Liang |
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| Institution: | Liang Beijing Insitute of Biotechnology,Beijing 100071,China |
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| Abstract: | Objective： To conjugate the virulent plasmid of Shigella flexneri 2a strain 2457T into Escherichia coli K12 strain MG1655.Methods： The mutant was constructed by the conjugation of the virulent plasmid of S.flexneri into MG1655,and the differentially expressed protein spots were identified by mass spectrum.Results： The mutant was successfully constructed,and the preliminary comparative proteomics analysis showed that certain virulent gene of S.flexneri 2a was expressed in the recombinant E.coli MG1655.Conclusion： The virulent plasmid of S.flexneri 2a strain 2457T has been successfully conjugated into E.coli K12 strain MG1655. |
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| Keywords: | Shigella flexneri virulent plasmid conjugation |
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