| 三倍体白杨杂种无性系指纹图谱的构建 |
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| 引用本文： | 尹敏娟,康向阳.三倍体白杨杂种无性系指纹图谱的构建[J].生物技术通讯,2012,23(1):86-89,147. |
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| 作者姓名： | 尹敏娟 康向阳 |
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| 作者单位： | 北京林业大学林木育种国家工程实验室,林木、花卉遗传育种教育部重点实验室,北京100083 |
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| 基金项目： | 林业公益性行业科研专项（201004009） |
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| 摘 要： | 目的:构建三倍体白杨杂种无性系指纹图谱,鉴定三倍体白杨杂种无性系。方法:分离纯化三倍体白杨杂种DNA模板,采用扩增片段长度多态性(AFLP)分子标记技术构建三倍体白杨杂种无性系指纹图谱。结果:从64对引物组合中筛选出M-CTA/E-CAG、M-CAC/E-CCA、M-ACT/E-CTC和M-CTT/E-CTG等4对多态性较高的引物组合,并应用该引物组合对21个三倍体白杨杂种无性系进行了AFLP分析,构建了21个三倍体白杨杂种无性系指纹图谱。结论:构建无性系指纹图谱是鉴别三倍体白杨杂种无性系的有效方法,能够有效鉴别21个三倍体白杨杂种无性系。本研究为品种鉴定及新品种权保护奠定了基础。
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| 关 键 词： | 白杨 杂种三倍体 扩增片段长度多态性标记 指纹图谱 品种鉴定 |
Construction of Fingerprinting Map of Triploid Hybrid Poplar Clones by AFLP Markers |
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| YIN Min-Juan
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KANG Xiang-Yang.Construction of Fingerprinting Map of Triploid Hybrid Poplar Clones by AFLP Markers[J].Letters in Biotechnology,2012,23(1):86-89,147. |
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| Authors: | YIN Min-Juan KANG Xiang-Yang |
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| Institution: | National Engineering Laboratory for Tree Breeding;Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants,Ministry of Education,Beijing Forestry University,Beijing 100083,China |
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| Abstract: | Objective： To identificate triploid hybrid clones of white poplar by fingerprinting map.Methods： The amplified fragment length polymorphism（AFLP） marker was conducted to construct fingerprinting map based on the isolation and purification of DNA.Results： The four pairs of primer combinations,M-CTA/E-CAG,M-CAC/E-CCA,M-ACT/E-CTC and M-CTT/E-CTG,which were of high polymorphic were screened out of 64 pairs of primers combinations.Then fingerprinting map of 21 triploid hybrid clones of white poplar was successfully constructed by AFLP markers.Conclusion： Fingerprinting map was one of the most effective methods to identificate hybrid clones by which 21 triploid hybrid clones were identificated.The related researches laid foundation for variety identification and plant variety protection. |
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| Keywords: | white poplar triploid hybrid clones amplified fragment length polymorphism marker fingerprinting map variety identification |
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