Isolated lymphoid follicles are not IgA inductive sites for recombinant Salmonella |
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Authors: | Hashizume Tomomi Momoi Fumiki Kurita-Ochiai Tomoko Kaminogawa Shuichi Hosono Akira Kataoka Kosuke Shinozaki-Kuwahara Noriko Kweon Mi-Na Yamamoto Masafumi |
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Institution: | Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, 2-870-1, Sakaecho-Nishi, Matsudo, Chiba 271-8587, Japan. |
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Abstract: | In this study, we investigated whether isolated lymphoid follicles (ILF) play a role in the regulation of intestinal IgA antibody (Ab) responses. The transfer of wild type (WT) bone marrow (BM) to lymphotoxin-alpha-deficient (LTalpha(-/-)) mice resulted in the formation of mature ILF containing T cells, B cells, and FDC clusters in the absence of mesenteric lymph nodes and Peyer's patches. Although the ILF restored total IgA Abs in the intestine, antigen (Ag)-specific IgA responses were not induced after oral immunization with recombinant Salmonella expressing fragment C of tetanus toxin. Moreover, Ag-specific cell proliferation was not detected in the ILF. Interestingly, no IgA anti-LPS Abs were detected in the fecal extracts of LTalpha(-/-) mice reconstituted with WT BM. On the basis of these findings, ILF can be presumed to play a role in the production of IgA Abs, but lymphoid nodules are not inductive sites for the regulation of Ag-specific intestinal IgA responses to recombinant Salmonella. |
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Keywords: | Isolated lymphoid follicles IgA Recombinant Salmonella |
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