Potential-dependent phase partitioning of fluorescent hydrophobic ions in phospholipid vesicles |
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Authors: | Douglas E Raines David S Cafiso |
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Institution: | (1) Department of Chemistry, University of Virginia, 22901 Charlottesville, Virginia |
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Abstract: | Summary Fluorescent, dansyl derivatives of triphenylalkylphosphonium ions have been synthesized and exhibit fluorescence intensities in small sonicated phospholipid vesicles that are dependent upon transmembrane potentials. The voltage-dependent fluorescence changes are a result of changes in quantum yield that accompany a voltage-dependent phase partitioning of the probe. This phase partitioning is easily quantitated by calibrating the intensities of totally membrane-associated and aqueous probe. The voltage-dependence is well accounted for by a simple thermodynamic model and allows an estimation of potentials from fluorescence intensities in small vesicle systems. |
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Keywords: | membrane potential hydrophobic ions fluorescent probes lipid vesicles |
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