The distribution of nuclear proteins and transcriptionally-active sequences in rat liver chromatin fractions |
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| Authors: | Nicolai M. Mironov Victor V. Lobanenkov Graham H. Goodwin |
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| Affiliation: | 1. INSERM, UMR1087, l''institut du thorax, CNRS, UMR6291, Université de Nantes, CHU de Nantes, Nantes F-44000, France;2. Department of Cardiology, Institute CARDIOMET, PCVM, University of Toulouse, CHU de Toulouse, France;3. Clinical Center of Investigation of Biotherapy, CIC, CHU Toulouse, France;4. INSERM, UMR1048 Institute of Cardiovascular and Metabolic Diseases (I2MC), Toulouse, France;5. French Blood Establishment, Midi-Pyrénées, Toulouse, France;6. Department of Cardiology, l''institut du thorax, Université de Nantes, CHU de Nantes, Nantes F-44000, France;7. Department of Hematology, CHU de Toulouse, France;8. Department of Imaging, CYCERON, CHU de Caen, France;9. Laboratoire d''épidémiologie et santé communautaire, UMR1027, Toulouse, France;10. Biologics Delivery Systems, Biosense Webster Inc. Johnson-Johnson, USA |
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| Abstract: | ![]()
Chromatin fractions from rat liver nuclei digested by nucleases were separated by differential solubility into several fractions. Material solubilized during digestion (predominantly monomer nucleosomes and polynucleosomes) had the highest HMG14 + 17/DNA ratios but were not enriched in active gene sequences (albumin and c-Ha-ras1 genes). Material soluble in a low ionic strength buffer containing 0.2 mM MgCl2 (monomer nucleosomes and polynucleosomes) contained in addition to the histones, HMG14 and 17 plus a 41K non-histone protein. This fraction was depleted in active gene sequences and enriched in inactive sequences. The insoluble material was highly enriched in active sequences and had the lowest HMG14 + 17/DNA ratio. This fraction could be further fractionated into a histone-containing 2 M NaCl-soluble fraction and a 2 M NaCl-insoluble matrix-bound fraction, both of which were enriched in active sequences. The results show that the HMG proteins do not partition with active sequences during fractionation of chromatin. The 41K protein may be associated with inactive chromatin fraction. |
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